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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Calcium-dependent cytosolic phospholipase A2 activation is implicated in neuroinflammation and oxidative stress associated with ApoE4

Fig. 4

Phosphorylated-cPLA2 levels are greater in the frontal cortex of persons with AD dementia with APOE3/E4 compared with APOE3/E3. The frozen inferior frontal lobe tissue from persons with NCI or AD dementia with different APOE genotypes were homogenized with RIPA buffer. cPLA2 was enriched by immunoprecipitation with anti-cPLA2 antibody and then the eluted complex was used to measure the phosphorylated cPLA2 (p-cPLA2) and total cPLA2 protein levels by Western blot. β-tubulin or β-actin were used as loading controls. A p-cPLA2 and cPLA2 protein levels in the cortex from persons with NCI (n = 6 for E3/E3, n = 6 for E3/4). B p-cPLA2 and cPLA2 protein levels in the cortex from persons with AD dementia (n = 6 for E3/E3, n = 8 for E3/E4). NCI, no cognitive impairment; AD, Alzheimer’s disease. Data are represented as mean ± SEM and analyzed by Student’s t-test (two-tailed). *p < 0.05

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Molecular Neurodegeneration

ISSN: 1750-1326