Fig. 3

Effect of human brain extract on spontaneous firing of cultured cortical neurons. A Time course of firing activity within rat cortical cultures prior to, during, and following treatment with human brain extract from 2 individuals. Each dot represents a single multi-electrode array (8 electrodes per array) during each recording. Solid line tracks the mean firing frequency per condition at each recording, and dashed lines indicate mean  ± SEM. B Comparison of firing frequencies across treatment conditions prior to, 4-days post-treatment, and 2-days following washout. Error bars represent mean  ± SEM. Significant differences between conditions indicated by brackets, *, p < 0.05, ***, p < 0.001. C Effects on spontaneous firing elicited across a panel of human brain extracts. An estimated effect on spontaneous firing of individual rat neurons at baseline (Day 0), 1-day, and 4-days following treatment with human brain extracts. Error bars represent mean  ± SEM as predicted by linear-mixed effect model based on treatment data from 19 individuals across 1 to 9 replicate experiments per individual. Numbers below indicate number of experiments (“N”) and number of independent arrays (“n”) tested for each brain sample