Fig. 4

Influence of human brain extracts on neurite integrity in vitro. A, B Representative images of human iPSC-derived neurons (iNs) treated with an LP-NCI brain extract (A) and neurons treated with AD brain extract (B) at 72 hr. post-treatment. Scale bars = 200 μm. C Quantification of data for each individual brain extract over 78 hours. Data show mean  ± SEM; n = 6 for each brain extract. D Estimated neurite integrity following 72-hr treatment by diagnosis class, combining all data from (C). N = 36 for vehicle-treated wells, and n = 36 for no treatment condition. Neurite integrity in human iNs normalized to baseline (time 0), examined at 2-hr intervals over 78-hr treatment with human brain extracts. One-way ANOVA with Tukey’s multiple comparisons test, comparing LP-NCI, HP-NCI, and AD-extract treated conditions. Significance is shown relative to the LP-NCI extract-treated condition. *, adjusted p < 0.005; **** p < 0.0001