Table 1 Primer pairs used for quantitative real-time PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
Gapdh | 5’- GGTGAAGGTCGGTGTGAACG-3′ | 5’- CTCGCTCCTGGAAGATGGTG-3′ |
Csf1 | 5’- AGTATTGCCAAGGAGGTGTCAG-3′, | 5’- ATCTGGCATGAAGTCTCCATTT-3′ |
Csf1r | 5’- GCAGTACCACCATCCACTTGTA-3′ | 5’- GTGAGACACTGTCCTTCAGTGC-3’ |
Itgax | 5’-ATGGAGCCTCAAGACAGGAC-3’ | 5’-GGATCTGGGATGCTGAAATC-3’ |
Mmp2 | 5’-GCTGTATTCCCGACCGTTGA-3’ | 5’-TGGTCCGCGTAAAGTATGGG-3’ |
Mmp12 | 5’-CTGCTCCCATGAATGACAGTG-3’ | 5’-AGTTGCTTCTAGCCCAAAGAAC-3’ |
Tnf-α | 5’-ATGAGCACAGAAAGCATGATCCGCG-3’ | 5’-CCCTTCACAGAGCAATGACTCCAAA -3’ |
Il-1β | 5’- ATGGCAACTGTTCCTGAACTCAACT-3’ | 5’- AGGACAGGTATAGATTCTTTCCTTT-3’ |
CD68 | 5’- TGTCTGATCTTGCTAGGACCG-3’ | 5’- GAGAGTAACGGCCTTTTTGTGA-3’ |
Axl | 5’- GGAGGAGCCTGAGGACAAAGC-3’ | 5’-GACAGCATCTTGAAGCCAGAGTAGG-3’ |
Cr3 | 5’- CGACACCATCGCATCTAA-3’ | 5’- TCCCTGAACATCACCACC-3’ |
Lpl | 5’- TTCCAGCCAGGATGCAACA-3’ | 5’- GGTCCACGTCTCCGAGTCC-3’ |
Apoc1 | 5’-TTCAGTTCGTGTGTGGACCGA-3’ | 5’- ATCCACAATGCCTGTCTGAGG-3’ |
Trem2 | 5’- TCATAGGGGCAAGACACCT-3’ | 5’- GCTGCTCATCTTACTCTTTGTC-3’ |
Tyrobp | 5’- GAGTGACACTTTCCCAAGATGC-3’ | 5’- CCTTGACCTCGGGAGACCA-3’ |
Aim2 | 5’- GTCACCAGTTCCTCAGTTGTG-3’ | 5’- CACCTCCATTGTCCCTGTTTTAT-3’ |
C3 | 5’- CCAGCTCCCCATTAGCTCTG-3’ | 5’- GCACTTGCCTCTTTAGGAAGTC-3’ |
Clec7a | 5’- GACTTCAGCACTCAAGACATCC-3’ | 5’- TTGTGTCGCCAAAATGCTAGG-3’ |
Serpina3n | 5’- ATTTGTCCCAATGTCTGCGAA-3’ | 5’- TGGCTATCTTGGCTATAAAGGGG-3’ |
Btk | 5’- AAGAAGCGCCTGTTTCTCTTG-3’ | 5’- GGTACGGGAACCTTTCAATGAT-3’ |
Cst7 | 5’- GGAGCTGTACTTGCCGAGC-3’ | 5’- CATGGGTGTCAGAAGTTAGGC-3’ |
Ccl2 | 5’- ATTCTGTGACCATCCCCTCAT-3’ | 5’- TGTATGTGCCTCTGAACCCAC-3’ |
Gpnmb | 5’- TGCCAAGCGATTTCGTGATGT-3’ | 5’- GCCACGTAATTGGTTGTGCTC-3’ |
B2m | 5’- TTCTGGTGCTTGTCTCACTGA-3’ | 5’- CAGTATGTTCGGCTTCCCATTC-3’ |
Ctsd | 5’- GCTTCCGGTCTTTGACAACCT-3’ | 5’- CACCAAGCATTAGTTCTCCTCC-3’ |