Fig. 3

Gene regulatory networks (GRN) of microglia demonstrate cuprizone induces a distinct state. a GRN of microglia and BAM clusters (b) from corpus callosum microglia isolated from naïve mice and mice fed cuprizone for five weeks. Legend represents the regulon specificity score with blue indicating higher Z-score values i.e., higher expression. c Unsupervised graph-based clustering onto a UMAP of SCENIC derived GRN scores and (d) gene expression from single cell RNA sequencing dataset highlighting three most enriched cuprizone associated microglia regulons: Atf3, Bhlhe40, and Hif1α. e Genes identified by SCENIC as being regulated by Atf3, Bhlhe40, and Hif1α