Fig. 4

FUS-ALS astrocytes fail to integrate in the motor neuron network. A Overview of coculture setup between hiPSC-derived motor neurons (MN) and astrocytes (AC). B Experimental assessment was performed in the highlighted motor neuron/astrocyte compartment of the microfluidic device after 1 and 2 weeks of coculture. C Scanning electron microscopy images of motor neuron and astrocyte cocultures in microfluidic devices after 1 week. Mutant astrocytes are circled with a white dashed line. Scale bar: 10 μm. D Top 25 RNAseq canonical pathways in mutant astrocytes compared to controls at week 4 of maturation. Analysis was performed with a cut-off Log Ratio of -1.0 to 1.0 and an FDR of 0.001. Dotted line marks p-value = 0.05. Cell illustrations are modified from Smart Servier Medical Art licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/). See also Suppl. Figure 6, Additional file 2