Fig. 1

Tamoxifen-induced Cre recombination reduces both APOE mRNA and protein. a Schematic timeline of tamoxifen treatment (5 daily injections at 200 mg/kg) in 2-month-old 5XFAD (line 7031) x APOE4^flox/flox (5X+AL-) or 5XFAD (line 7031) x APOE4^flox/flox x Aldhl1l-Cre/ERT2 (5X+AL+) mice, assessed at 10-months of age. b Relative expression of APOE mRNA normalized to beta-actin in cortex. c, d PBS-soluble and guanidine-HCL-soluble (“insoluble”) APOE protein concentrations assessed by ELISA from cortex. Data expressed as mean ± SD, student’s t-test (two-sided) performed for all statistical analyses except (b), where Welch’s t-test was performed. ∆ = males, ○ = females. ***P < 0.001, ****P < 0.0001. No other statistical comparisons are significant unless indicated