Fig. 2

Removal of astrocytic APOE4 before amyloid deposition shifts Aβ distribution from plaques to the cerebrovasculature. a–d, X34 staining for fibrillar plaques/CAA (a) with percent area coverage of total X34 (b), CAA (c), and amyloid plaques (d) in the cortex overlying the hippocampus of 10-month-old 5X+AL- or 5X+AL+ mice after astrocytic APOE4 removal at 2-months-of-age. e, Proportion of CAA in total X34⁺ staining. f–i, Aβ immunoreactivity (Aβ-IR) (f) with percent area coverage of total Aβ-IR (g), vascular Aβ-IR (h), and plaques (i) in the cortex overlying the hippocampus. j Proportion of vascular Aβ in total Aβ-IR. Scalebar: 300 µm. Data expressed as mean ± SD, unpaired student’s t-test (two-sided) performed for all statistical analyses except (b), where Welch’s t-test was performed. ∆ = males, ○ = females. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. No other statistical comparisons are significant unless indicated