Fig. 4

Depletion of APOE in astrocytes diminishes certain disease-associated glial signatures. a–e, GFAP⁺ astrocytic staining (a) and quantification of area coverage (b) in the cortex of 10-month-old 5X+AL- or 5X+AL+ mice. Scalebar: 300 µm. Representative images (c) and quantification of the number of astrocytes surrounding CAA (d) or plaques (e). Scalebar: 20 µm. Each point represents the number of astrocytes surrounding a single CAA or plaque normalized to that CAA or plaque area from n = 8–10 mice per group. f–j, IBA1⁺ microglial coverage (f) and quantification of area coverage (g) in the cortex overlying the hippocampus. Scalebar: 300 µm. Per plaque analysis of number of microglia clustering CAA (h, i) or plaques (j). Scalebar: 20 µm. Each point represents the number of microglia surrounding a single CAA or plaque normalized to that CAA or plaque area from n = 8–10 mice per group. k, l, Relative mRNA expression of homeostatic and disease-associated astrocytic (k) and microglial genes (l). Data imputed for Clec7a mRNA (column 10) but not used in statistical analysis. Each column represents an individual mouse. Data expressed as mean ± SD, student’s t-test (two-sided) performed for all statistical analyses except (d), (e), (k – S100β), and (l – Cst7, Cst3, Itgax, Spp1) where Welch’s t-test was performed. ∆ = males, ○ = females. *P < 0.05, **P < 0.01. No other statistical comparisons are significant unless indicated