Fig. 9
From: BACE1 regulates expression of Clusterin in astrocytes for enhancing clearance of β-amyloid peptides
Targeted astrocytic deletion of Bace1 reduces amyloid plaques by increasing Aβ clearance. A Thioflavin-S staining of amyloid plaques from saggital brain sections of 5xFAD;Bace1fl/fl;Gfap-cre and 5xFAD;Bace1fl/fl. Scale bar indicates 100 µm (B) Quantification of Thioflavin-S positive amyloid plaques calculated by counting serial sagittal sections, which were selected at 10-section intervals (N = 7 for 5xFAD;Bace1fl/fl;Gfap-cre and 5xFAD;Bace1fl/fl). C Western blot of cortical lysates from 5xFAD;Bace1fl/fl;Gfap-cre and 5xFAD;Bace1fl/fl mice. Images indicate APP full length, APP C-terminal C99 (detected by 6E10) and C99/C83 bands (detected by APP A8717 antibody). Actin was included as loading control