Fig. 8
HDL and LDL significantly modulate α-syn aggregation in SAAs. A-B Representative SAAs traces performed on a neat PD (PD47) CSF sample and on a neat HC (HC22) CSF sample (1 × physiological concentration) and the same samples spiked with 0.006 mg/mL (2 × physiological concentration) and 0.024 mg/mL (5 × physiological concentration) HDL or LDL. The average kinetic traces with error bars representing the SEM calculated on three replicates of wells containing CSF additioned with HDL and LDL are shown in panels A and B, respectively. C-D Average time-to-threshold (TTT) values measured in all the PD samples. The average TTT and SEM were calculated by assuming a TTT of 125 h (maximum TTT observed) for replicates in which aggregation was not considered significant (Fmax < 5000 a.u.). One-way ANOVA coupled with Tukey post-hoc test was applied to assess the statistical significance of the observed relative differences of all the individual measured traces among neat, 2 × HDL/LDL and 5 × HDL/LDL. Significant differences were marked with * with *** indicating a p-value < < 0.001. D Summary of the final SAA outcome for the analysed PD and HC samples. The outcome was categorized as: positive ( +) when 3/3 replicates were determined positive by the probabilistic algorithm, inconclusive (?) when 2/3 replicates were determined positive by the probabilistic algorithm, and negative (-) when just 1/3 or 0/3 replicates were determined positive by the probabilistic algorithm