Fig. 3

Cholinergic accumulation of hTau inhibits asymmetrical discharges with no influence on the symmetrical currents. A Representative image showing hTau accumulation in cholinergic neurons. Scale bar, 50 μm. B Two discharge waveforms recorded in cholinergic neurons: asymmetric (top) and symmetrical (bottom). The baseline membrane potential is -65 mV. C, D MS-ChAT-hTau mice showed a significantly decreased excitability in asymmetrically discharged cholinergic neurons (C two–way ANOVA group × current, frequency: F [9,220] = 2.758, P < 0.01) with no significant changes in the symmetrically discharged cholinergic neurons (D two–way ANOVA group × current, frequency: F [9,140] = 0.07977, P > 0.05). E–H No differences in amplitude (E asymmetrically, unpaired t test, t = 1.596 df = 22, P > 0.05; symmetrically, unpaired t test, t = 0.02285 df = 14, P > 0.05), CM (F asymmetrically, unpaired t test, t = 0.7843 df = 22, P > 0.05; symmetrically, unpaired t test, t = 0.3898 df = 14, P > 0.05), half-width (G asymmetrically, unpaired t test, t = 1.832 df = 22, P > 0.05; symmetrically, unpaired t test, t = 1.785 df = 14, P > 0.05), and RMP (H asymmetrically, unpaired t test, t = 0.5846 df = 22, P > 0.05; symmetrically, unpaired t test, t = 2.002 df = 14, P > 0.05) were detected between MS-ChAT-hTau group and the controls in neither asymmetrically- nor symmetrically- discharged cholinergic neurons in the MS. N = 5 mice per group (12 recordings in asymmetrically discharged cholinergic neuron and 8 recordings in symmetrically discharged cholinergic neuron). *P < 0.05, **P < 0.01 vs control group