Fig. 6

Cholinergic-specific overexpressing hTau disrupts theta rhythm in the MS and its synchronization with CA1. A, B Diagram and schematics shown local field potential signal recording in the MS and CA1. C Nissl staining confirmed electrode position in the MS and the unilateral CA1. D-F Representative heatmap of MS and dCA1 shown decreased power of theta rhythm measured before, during and after MBM test in MS-ChAT-hTau mice as compared with the control group. G-I Representative curves shown decreased coherence recorded in MS and CA1 of a MS-ChAT-hTau mouse and its control before, during and after MBM test. J-O Cholinergic-specific overexpressing hTau decreased theta power measured during (at 4–7 Hz and 8–14 Hz) and after (8–14 Hz) MBM test. J 1–3 Hz, unpaired t test, pre, t = 0.5813, df = 12, P > 0.05; in, t = 0.2973 df = 12, P > 0.05; post, t = 0.9549, df = 12, P > 0.05. K 4–7 Hz, unpaired t test, pre, t = 1.269, df = 12, P > 0.05; in, t = 2.378, df = 12, P < 0.05; post, t = 2.096, df = 12, P > 0.05. L 8 -14 Hz, unpaired t test, pre, t = 1.961 df = 12, P < 0.05; in, t = 2.262, df = 12, P < 0.05; post, t = 2.352, df = 12, P < 0.05. M 15–29 Hz, unpaired t test, pre, t = 0.4238, df = 12, P > 0.05; in, t = 0.4707, df = 12, P > 0.05; post, t = 1.684, df = 12, P > 0.05. N 30–49 Hz, unpaired t test, pre, t = 0.9446, df = 12, P > 0.05; in, t = 0.2927, df = 12, P > 0.05; post, t = 0.04679, df = 12, P > 0.05. O 50–100 Hz, unpaired t test, pre, t = 0.7450 df = 12, P > 0.05; in, t = 0.1598, df = 12, P > 0.05; post, t = 0.1340, df = 12, P > 0.05. P-U Cholinergic-specific overexpressing hTau did not significantly affect PSD in the CA1 at different frequency bands before, during and after BM test. P 1–3 Hz, unpaired t test, pre, t = 0.8001, df = 12, P > 0.05; in, t = 0.5970, df = 12, P > 0.05; post, t = 0.4382, df = 12, P > 0.05. Q 4–7 Hz, unpaired t test, pre, t = 1.245, df = 12, P > 0.05; in, t = 0.7448, df = 12, P > 0.05; post, t = 0.6663, df = 12, P > 0.05. R 8 -14 Hz, unpaired t test, pre, t = 0.9599 df = 12, P > 0.05; in, t = 0.7542, df = 12, P > 0.05; post, t = 0.4606, df = 12, P > 0.05. S 15–29 Hz, unpaired t test, pre, t = 0.7957, df = 12, P > 0.05; in, t = 0.4421, df = 12, P > 0.05; post, t = 0.6501, df = 12, P > 0.05. T 30–49 Hz, unpaired t test, pre, t = 0.6279, df = 12, P > 0.05; in, t = 0.3543, df = 12, P > 0.05; post, t = 0.6380, df = 12, P > 0.05. U 50–100 Hz, unpaired t test, pre, t = 0.3743, df = 12, P > 0.05; in, t = 0.3152, df = 12, P > 0.05; post, t = 0.5692 df = 12, P > 0.05. V-X Cholinergic-specific overexpressing hTau specifically disrupted theta coherence between MS and CA1 measured before, during and after BM test. V coherence, two–way ANOVA group × frequency, F [5,72] = 5.634, P < 0.01; W coherence, two–way ANOVA group × frequency, F [5,72] = 2.851, P < 0.05; X coherence, two–way ANOVA group × frequency, F [5,72] = 2.495, P < 0.05. N = 7 mice per group. *P > 0.05, ** P > 0.01. Data were presented as mean ± SEM