Fig. 3
From: Sex specific molecular networks and key drivers of Alzheimer’s disease
LDLR-related protein 10 (LRP10) Identified and Validated as a Sex-specific Key Regulator of AD. A LRP10-centered 2-layer networks constructed with the female AD samples (Left) and the male AD samples (Right). The pie chart of each node indicates whether it is a DEG identified between AD and the control (upper half) or between women and men (lower half). Warm colors in the pie chart represent the upregulation of the gene in AD/women; cool colors in the pie chart represent the downregulation of the gene in AD/women. Genes with blue labels are AD risk genes from Alzgen. B Procedure of functional validation of LRP10. Levels of C lrp10 mRNA by qPCR analysis (data presented as log2FC) and D LRP10 protein by western blot (data presented as log2FC) were compared between AD versus control, male versus female, APOE4 carriers (APOE4+/-) versus non carriers (APOE3+/+) in the PHG human brain samples. ANOVA with post-hoc tests to determine group differences for multiple comparisons and independent-samples t-tests for paired comparisons. ****p < 0.0001; ***p < 0.001; *p < 0.05. E The correlation of qPCR analysis (lrp10 mRNA) versus western blot analysis (LRP10 protein) of the PHG human samples were shown