Fig. 2

The decrease in hippocampal expression of ACSS2 is associated with cognitive impairment in middle-aged 5 × FAD mice. A Representative immunoblots and quantitative analyses of ACSS2 in the hippocampi of the 2-, 5-, 8- and 10-month-old WT or 5 × FAD mice (n = 6 per group). B-E The 8-month-old WT and 5 × FAD mice were examined by the Morris water maze (MWM) test. Escape latency (B) to the platform position during the training trials (1-5d) and the probe (6d) trial. The number of platform-position crossings (C), the percentage of time spent in the target quadrant (D), and the speed (E) in the probe (6d) trial. n = 15 and 12 mice for 8-month-old WT and 5 × FAD, respectively. F Linear regression demonstrated a positive correlation of ACSS2 protein level with the percentage of time spent in the target quadrant (F, left panel) or the number of platform-position crossings (F, right panel) in the MWM test (n = 6 mice/group). Data are expressed as mean ± SEM. Statistical significance was calculated by unpaired Mann-Whitney test (C), unpaired two-tailed t-test (D-E), linear regression analysis (F), one-way ANOVA (A), and two-way ANOVA (B) followed by the Tukey’s post-test. * P < 0.05, ** P < 0.01, *** P < 0.001