Fig. 3

Ageing affects the succinate, tryptophan and tyrosine metabolites in the feces and serum. Metabolites combined fold change (FC > 1.2 (log2 FC)) and p-value < 0.05 (-log10 (p value)) volcano plot analysis identified effect of ageing between WT and TG rat feces and serum. a At 3 M age feces genotype comparison TG/WT phenylacetate is significantly upregulated whereas at > 12 M age 4-Hydroxyphenylacetate and lactate were up while glutamate were down. b In serum samples glutamate is significantly decreased at 3 M whereas at > 12 M succinate, glutamate and lactate are increased. c When only observing the ageing effect on TG serum metabolites, succinate is significantly upregulated, and succinate correlation coefficient analysis suggested that tryptophan and tyrosine were negatively correlated with succinate while other short chain fatty acids were positively correlated. Statistically significant levels showed in the respective volcano and violin plots. P value significance represents *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001