Fig. 5

Increased transepithelial current in TG rats. a A schematic diagram showing the ‘Ussing chamber’ technique to measure the function of intestinal permeability and Na ⁺ uptake to gut epithelial cells (ENaC channel). b Original tracing illustrating the effect of currents (1 µA) and of amiloride (50 µM) on transepithelial potential across colonic epithelium from WT (upper) and TG (lower) from 2 and 12 M rats (N = 3–5 female/genotype). c Arithmetic mean ± SEM (N = 3–5/group) of amiloride-sensitive current across colonic epithelium (Na⁺ absorption) from TG and WT rats and resistance of colonic epithelium (permeability). d Fecal hypoxanthine levels measured by ¹H-NMR from 3 M and > 12 M WT and TG rats. P value significance represents *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ***p ≤ 0.001