Fig. 1

Biosensor probe design affects detection of bioactive tau seeds. A. Schematic of the various tau probes that were designed. Tau sequence (244:378) consisted in the repeat domain and amino acids included in the core of AD tau filaments. Variations in the linker sequence composition between tau sequence and fluorescent reporter were introduced in construct. 3 KQ mutations were also introduced to mimic posttranslational modifications. B. Tau seeding was quantified by flow cytometry on cells transiently transfected with constructs containing variations in the linker sequence and exposed to serial dilutions of AD brain lysate (left panel). The right panel represents the data extracted for a tau concentration of 20 ng..C. Similar experiment performed comparing the effect of 3xKQ modifications on tau sequence. D. Schematic of the lentiviral vectors that were produced to generate stable cell lines. E. Stable cell populations generated by lentiviral transduction were compared to the original biosensor line in a flow-cytometry based seeding assay. Each point represents mean of 4 biological replicates. Error bars at standard error of the mean