Fig. 2

Novel biosensor HEK293T clone 18 demonstrates increased sensitivity in amplifying bioactive tau seeds. A. The selected biosensor clone was compared to the original tau biosensor cell line in a flow-cytometry based seeding assay after exposure to serial dilutions of total tau in human AD brain lysate or control brain lysate. B. Expansion of A in the lower tau concentration range. Each point represents mean of 4 biological replicates. Error bars at standard error of the mean. The table summarize the calculated lower limit of detection. Values are means of 2 independent experiments ± standard deviation. C. Fluorescence imaging of the selected biosensor cell clone 24 h after seeding with AD brain lysate, depicting representative fibrillar structure of aggregated tau probe. Lower panels depict the same condition with higher magnification. D. Representative image of tau nuclear speckles detected after seeding in new biosensor cell clone. E. Detection of brain-derived human tau-13 positive inclusions (arrowheads) embedded in fibrillar tau probe aggregates 24 h after seeding. Scale bar is 10 μm