Fig. 3

Tau probe in biosensor cells aggregates in insoluble beta pleated structures after seeding. A. Schematic of insoluble and soluble tau extraction protocol after seeding. B. Western blot comparing tau probe expression in the original biosensor cell line and novel biosensor HEK293T clone 18 after seeding in soluble and sarkosyl insoluble fractions. An antibody recognizing the repeat domain of tau was used as a primary antibody. GAPDH was used as a loading control. Uncropped images of western blot membranes are displayed in Supplementary Fig. 2. Thiazine red staining was performed to detect beta-pleated conformation 24 h after seeding with AD brain lysate or control brain lysate in HEK293T novel biosensor clone (C) and original biosensor line D. Scale bar is 10 μm