Fig. 1

KDS-5104 activates PPARα and TFEB signaling in microglial cells. (a) qPCR analysis of expression of Ppara and downstream target Cyp4a14 in sorted microglia isolated from brains of 9-month-old 5xFAD and WT littermates (n = 5/group). (b) qPCR analysis of Tfeb and downstream target Mcoln1 of the same samples as a) (n = 5/group). c. qPCR analysis of PPARA and CYP4A11 expression in KDS-5104 treated WT and TKO HeLa cells (n = 9/condition). d. qPCR analysis of Tfeb and Mcoln1 in WT and PPARαKO primary microglial cultures (n = 8/condition). e. qPCR analysis of Tfeb and Mcoln1 in vehicle or KDS-5104 treated BV2 cells with or without pretreatment with PPARα antagonist GW6471 (n = 8/condition). f. Model of KDS-5104 action of PPARα activation and TFEB signaling. For all panels, data are presented as mean ± SEM. ns: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001 by 2-sided t tests (a, b) or one way ANOVA with Tukey’s multiple comparisons tests as the post hoc analysis (c-e)