Fig. 2

KDS-5104 increases TFEB lysosomal genes independent of mTORC1. (a) qPCR analysis of lysosome enzyme genes LAMP1, NEU1, and NAGLU in vehicle or KDS-5104 treated WT and TKO cells (n = 8/condition). (b) qPCR analysis of Lamp1, Neu1, Naglu in vehicle or KDS-5104 treated WT and PPARαKO primary microglia cultures (n = 10/condition). (c) Western blot analysis of total and phosphorylated S6K (pS6K) levels in primary microglial cultures treated with vehicle (Control), KDS-5104 (10 µM) or Torin (500 nM). γ-tubulin was used as a loading control. (d) Quantification of pS6k expression level normalized to γ-tubulin (left) and the ratio of pS6k to total S6k (right) (n = 6/condition). AU: artificial unit. For all panels, data are presented as mean ± SEM. ns: non-significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. One way ANOVA with Tukey’s multiple comparisons tests as the post hoc analysis