Fig. 7

Reduced reactive gliosis and Aβ pathology in 5xFAD mice treated with KDS-5104. (a) Representative images of GFAP (magenta) and Iba1 (yellow) co-staining from the hippocampal sections of 4-month-old 5xFAD and WT mice treated with vehicle or 10 mg/kg KDS-5104 for two months. Scale bar:. (b) Quantification of Iba1 and GFAP area fluorescence (n = 5–6 mice/group). (c) Representative images of 6E10 (white) of hippocampal sections of 4-month-old 5xFAD treated with vehicle or KDS-5104. (d) Quantification of 6E10 positive area and plaque number (n = 5–6 mice/group). (e) Representative images of CD68 (red), Iba1 (yellow) and 6E10 (white) co-staining and 3D rendering created by Imaris imaging software. f-h. Quantification of plaque size (f), CD68 fluorescent intensity (g), microglia per plaque (h) and microglia area (i)(n = 5–6 mice/group). AU: artificial unit. Scale bar: 100 μm in (a) and (c) and 10 μm in (e). For all panels, data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one way ANOVA with Tukey’s multiple comparisons tests as the post hoc analysis (panel b) or 2-sided t-tests (panels d and f-i)