Fig. 8
Improved synaptic properties and cognitive performance in 5xFAD mice treated with KDS-5104. (a) Representative images of co-staining of post-synaptic marker PSD95 and presynaptic marker synaptophysin (Syp) from hippocampal CA1 area of 4-month-old WT and 5xFAD mice treated with vehicle or 10 mg/kg KDS-5104. Inset showing enlarged view of Syp and PSD95 co-localized puncta. Scale bar: 100 μm; 25 μm zoom in. (b) Quantification of the number of PSD95, Syp and co-localized Syp and PSD95 puncta (n = 6 mice/group). (c) Quantification of percent time exploring the novel object in NOR assay. (d) Freezing percentage due to contextual or cue testing in the fear conditioning paradigm (n = 11–13 mice/group). For all panels, data are presented as mean ± SEM. ns: non- significant, #p = 0.11, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. One way ANOVA with Tukey’s multiple comparisons tests as the post hoc analysis