Fig. 1

3D6 treatment induces microhemorrhages in PDAPP mice. (a) Prussian Blue (hemosiderin, blue) labeled microhemorrhages in the leptomeninges and penetrating vessels of PDAPP mice treated with IgG or 3D6. (b) Quantification of Prussian blue + area (%) in brain coronal sections of IgG or 3D6-treated mice. (c) Measurement of the diameter (dashed line) of the leptomeninges in mice treated with IgG or 3D6. For quantifications, total coronal sections were used, and each data point indicates an animal n = 20–25 (mice). All are representative images of 26-month-old PDAPP mice. Results are shown as ± SEM, asterisks indicate significant differences, where **p < 0.01 and ***p < 0.001 by unpaired Student’s t test. Scale bar 40 μm