Fig. 1

Apoer2-ICD regulation of translating transcripts in vivo. A Rosa^26fs-TRAP mice crossed with the Apoer2^WT, conditional KO, and cleavage-resistant Apoer2 transgenic mice (Apoer2^Δ16±19) were injected with lentiviruses expressing Cre resulting in a GFP-tagged ribosomal subunit (L10a:GFP) to allow for pull-down of ribosome-bound transcripts (intrahippocampal injections; coordinates were AP: -2.2, ML: ±1.3, DV, -1.3). Using an Internal Ribosome Entry Site (IRES), Apoer2-ICD^[±19] was co-expressed to assess the effect of overexpression of either Apoer2-ICD in Apoer2^WT or rescue of effects of lack of the Apoer2-ICD (B). C-D Heatmaps representing the proportion of genes altered in Apoer2 transgenic mice that are either rescued or not rescued with the Apoer2-ICD[±19] (C) or altered by overexpression of either Apoer2-ICD in Apoer2^WT or (D) effects of the ICD independent of the lack of ICD-release. E Enrichment analysis of the ~4700 transcripts differentially translated across all conditions, demonstrating enrichment for synaptic compartments, neuronal processes and pathways, as well as diseases of the brain. F Gene-term network of the ClueGO/Cluepedia enrichment analysis of synaptic transcripts annotated in the SynGO database. (For each mouse line: Cre-only and Cre+Apoer2-ICD[+19]: n= 4 individual and one pooled set of 4 RNA samples, Cre+Apoer2-ICD[Δ19]: n= 4 individual RNA samples)