Fig. 4

Identification of six microglial activation-dependent proteins. (A) Log2 transformed abundances of panel 6 proteins (FABP3, MDH1, GDI1, CAPG, CD44, and GPNMB) in the secretome of GRN ko hiMGL (pink) and in CSF of symptomatic FTD-GRN patients (orange). The abundance of each group is normalized to the abundances measured in control groups, secretome of WT hiMGL and CSF levels of healthy non-carrier (CON). (B) Binary representation of the up- (red) and downregulation (blue) of each protein in hCSF of FTD-GRN patients, conditioned media of GRN ko hiMGL, cell lysates of GRN ko hiMGL, CSF (mCSF) of Grn ko mice, and microglia (mMG) of Grn ko mice. Crosses indicate missing values. (C) Comparison of Log2 transformed abundance of panel 6 proteins in CSF from FTD-GRN patients, conditioned media from GRN ko hiMGL, GRN ko hiMGL cell lysates, CSF and microglia from Grn ko mice. Circle size indicates statistical significance based on p-value obtained from Student’s t-test, * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001, n.s. = non-significant. False discovery rate (FDR) was not considered for the presented visualizations. Color intensity indicates the degree of up- (red) or downregulation (blue). Unchanged abundances are indicated in gray and are defined by -0.2 < Log2 fold change < 0.2