Fig. 6
CSF levels of FABP3, MDH1, and GDI1 correlates with each other and are significantly elevated in an amyloid dependent manner in MCI and AD. (A) Overview of the EMIF-AD MBD cohort with stratification according to diagnosis only. (B) CSF levels of FABP3 (top), MDH1 (middle), and GDI1 (bottom) in cognitively normal controls (CNC) (gray, n = 126), SCI (blue, n = 61), MCI (pink, n = 198), and AD (yellow, n = 93). CSF levels are defined by Log2 abundance, normalized to the abundance measured in the TMT-reference control channel [15] and the median of each individual. (C) Overview of the EMIF-AD MBD cohort with stratification according to diagnosis and amyloid status as assessed by the levels of CSF amyloid-β1−42[22]. (D) CSF levels of FABP3 (top), MDH1 (middle), and GDI1 (bottom) in CNC A− (light gray, n = 70), CNC A+ (dark gray, n = 18), SCI A− (light blue, n = 34), SCI A+ (dark blue, n = 27), MCI A− (light pink, n = 104), MCI A+ (dark pink, n = 93), AD A− (light yellow, n = 11), and AD A+ (dark yellow, n = 82). A− = amyloid normal, A+ = amyloid abnormal [15]. Ordinary one-way ANOVA (alpha = 0.05). (E-F) ROC curve analysis indicating the diagnostic performance of panel 6 (AUC = 0.94, 95% CI = 0.87 to 1.0) to separate cohort participants as affected (amyloid-positive AD) versus not affected (amyloid-negative CNC). (G-H) Correlation analysis of panel 6 proteins and TREM2 (microgliosis), GFAP (astrogliosis), and CHI3L1 (also known as YKL-40, neuroinflammation) in (G) amyloid-negative CNC and in (H) amyloid-positive AD patients. CSF levels are defined by Log2 abundance, normalized to the abundance measured in the TMT-reference control channel and the median of each individual. Each cell is colored and labeled according to Spearman r value; Spearman r > 0.5 is normally considered to indicate a strong correlation, while Spearman r > 0.75 is considered to indicate a very strong correlation. * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001, n.s. = non-significant. FDR was not considered for the presented visualizations