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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps

Fig. 5

Translocated BAX exhibits a delay before being activated. A,B Image of 2 cells exhibiting punctate localization of GFP-BAX induced by ONC and counterstained with the 6A7 monoclonal antibody that recognizes an epitope of BAX that is exposed after activation. Only 1 of the 2 cells is clearly labeled by the antibody. Scale bar = 15 µm. C Quantification of the percentage of punctate GFP-BAX cells that are negative for 6A7-staining at times after optic nerve crush (PostONC). The mean percentages of cells that are paused for GFP-BAX activation are 48.27% at 3 days after ONC, which decreases to 39.51% at 5 days and 24.73% at 7 days. D Scatter plot showing the percentage of cells with punctate mCherry-BAX that also have punctate cytochrome c (Cyt C)-GFP (see Supplemental Figure S4) at days 3, 5, and 7 after ONC. The percentage of paused cells is 50.91% at 3 days after ONC and drops to 38.01% and 25.16% on days 5 and 7, respectively. (*P < 0.001, ** P = 0.0025, *** P < 0.0001)

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Molecular Neurodegeneration

ISSN: 1750-1326