Table 1 List of human iPSC lines used in this study
Cell line | Description | Source |
|---|---|---|
WT | WTC 11 line, Healthy 30-year-old Japanese male donor | Coriell Institute #GM25256 |
GRN KO | WTC11 line with a 7 base pair insertion in one GRN allele and 10 base pair deletion in the other GRN allele resulting in complete loss of function | Generated in house |
ptMut | FTD patient cell line harboring a heterozygous GRN mutation (c.26 C > A, p.A9D) | Dr. Dimitri Krainc [35] |
ptWT | Isogenic control line by correcting the GRN mutation in ptMut line | Dr. Dimitri Krainc [35] |
ptKO | Complete knock out of GRN in ptWT line using CRISPR-Cas9 | Generated in house |