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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Sleep restoration by optogenetic targeting of GABAergic neurons reprograms microglia and ameliorates pathological phenotypes in an Alzheimer’s disease model

Fig. 3

Effect of chronic optogenetic stimulation of GABAergic neurons on amyloid plaque deposition in APP mice. (A and B) Experimental design. After AAV infusion and cannula installation, mice received 1-month of continuous optogenetic stimulation. Multiphoton imaging (B) was performed after treatment. (C) Representative multiphoton images of Methoxy-X04 positive amyloid plaques (Cyan) and blood vessels (Red) in APP mice in absence of stimulation (APP-mCherry-no opto), in presence of stimulation of mCherry (APP-mCherry-opto) and during stimulation of ChR2 (APP-ChR2-opto). (D) Amyloid plaque number across conditions. (E) Amyloid plaque size across conditions. (F) Amyloid plaque burden across conditions. (G) Representative images of 6E10, 82E1 and Methoxy-X04 positive amyloid plaques in cortex and hippocampus within postmortem sections. (H-J) 6E10 (H), 82E1 (I) and Methoxy-X04 (J) positive amyloid plaque burden across conditions. All data are expressed as means ± standard error. The number of mice examined: n = 6–7 mice/group. *P < 0.05, **P < 0.01. n.s. not significant. Scale bars: 50 μm

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Molecular Neurodegeneration

ISSN: 1750-1326