Fig. 1

AAV-PHP.eB hα-syn transduces several cerebral regions and induces widespread expression of the transgenes in the brain 2-weeks post-AAV delivery. (A-B) Representative confocal microscopy images of sagittal brain slices of mice overexpressing (A) hα-syn or (B) mTurq (scale bar = 1 mm). Numbered boxes represent zoomed images of the different brain regions: [1] cortex, [2] striatum, [3] hippocampus, [4] thalamus, [5] midbrain, [6] hypothalamus, and [7] cerebellum (scale bar = 10 μm). (C-F) Western blot analysis of hα-syn, mα-syn and mTurq protein levels in the soluble protein fraction from the (C) substantia nigra, (D) striatum, (E) motor cortex, and (F) hippocampus. GAPDH was used as a loading control. (G-H) Quantification of the levels of (G) hα-syn and (H) mTurq in different brain regions normalized to GAPDH. (I) Ratio of hα-syn protein levels compared to endogenous mα-syn. The data are expressed as the means ± s.e.m. (n = 4–5 mice per experimental condition). SN: substantia nigra, Str: striatum, Ctx: cortex, Hip: hippocampus. Full Western blots are shown in Suppl. Figure 8