Fig. 5

Overexpression of hα-syn induces a neuroinflammatory response in the midbrain. (A) Representative fluorescence confocal microscopy images of coronal brain slices illustrating Iba1 staining in different brain regions (cortex, striatum, hippocampus, and SN) (scale bar = 20 μm). (B) Quantification of total processes length of microglia in different brain regions of mice overexpressing mTurq or hα-syn, 3-months post-AAV delivery. (C) Quantification of total processes length of microglia in the SN of mice overexpressing mTurq or hα-syn, 2-weeks and 3-months post-AAV delivery. (D) Quantification of the number of Iba1⁺ cells in different brain regions of mice overexpressing mTurq or hα-syn, 3-months post-AAV delivery. (E) Quantification of the number of Iba1⁺ cells in the SN of mice overexpressing mTurq or hα-syn, 2-weeks and 3-months post-AAV delivery. (F) Quantification of the percentage area of GFAP staining in different brain regions of mice overexpressing mTurq or hα-syn, 3-months post-AAV delivery. (G) Quantification of the mean intensity of GFAP signal/staining in different brain regions of mice overexpressing mTurq or hα-syn, 3-months post-AAV delivery. The data are presented as the means ± s.e.m. (n = 5–6 mice per experimental condition). (B, D, G & F) Two-way ANOVA followed by Tukey’s multiple comparisons test; * p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. (C & E) One-way ANOVA followed by Tukey’s multiple comparison test; ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001. Ctx: cortex; Str: striatum; Hip: hippocampus; SN: substantia nigra