Table 1 List of the antibodies used in this study

Primary antibodies

α-syn/ mouse

Syn1 / 610,787

15–123

1:1000

1:1000

BD Laboratory

(Billerica, MA, USA)

Human α-syn/ mouse

LB509 / 180,215

115–122

1:500

ThermoFisher

Scientific (Waltham,

MA, USA)

α-syn/ rabbit

Syn FL-140 / sc-10,717

65–91

1:1000

Santa Cruz Biotech

(Dallas, TX, USA)

α-syn/ rabbit

Anti-Alpha-synuclein antibody [MJFR1]/ (ab138501)

118–123

1:1000

Abcam (Cambridge,

UK)

pS129 α-Syn/ mouse

WAKO / pSyn #64

Phospho-Ser129

1:1000

1:2000

WAKO (Richmond,

VA, USA)

pS129 α-Syn/rabbit

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]/ Ab51253

Phospho-Ser129

1:1000

Abcam (Cambridge,

UK)

pS129 α-Syn/mouse

pS129 (Ghanem et al., 2022)

Phospho-Ser129

100 ng/ml

Ghanem et al., 2022

Beta-actin/ mouse

β-actin clone BA3R / G043

Beta-actin N-terminal peptide-KLH conjugates.

1:10000

Abm (Vancouver, BC, Canada)

Drebrin/mouse

Drebrin clone MX823/612,128

 C-terminal 632–649

coupled to KLH

1:1000

Progen (Heidelberg, Germany)

GAPDH/ mouse

GAPDH loading control/G041

–

1:2500

Abm (Vancouver, BC, Canada)

GFP/ rabbit

GFP/A-6455

Full length protein

1:5000

ThermoFisher

Scientific (Waltham,

MA, USA)

Non-phosphorylated α-syn/ mouse

4B1

125–133

100 ng/ml

Ghanem et al., 2022

Full-length α-syn aggregates /mouse

Syn-O2

100 ng/ml

Ghanem et al., 2022

Full-length α-syn aggregates /mouse

Syn-O1

100 ng/ml

Ghanem et al., 2022

Full-length α-syn aggregates /mouse

Syn-O3

100 ng/ml

Ghanem et al., 2022

Full-length α-syn aggregates /mouse

Syn-F1

100 ng/ml

Ghanem et al., 2022

Full-length α-syn aggregates /mouse

Syn-F2

100 ng/ml

Ghanem et al., 2022

GFAP/ rabbit

anti-Glial Fibrillary Acidic Protein / Z0334

Full length protein

1:800

Dako (Santa Clara, CA, USA)

GFAP/ mouse

Anti-Glial Fibrillary Acidic Protein

clone GA5/ MAB360

Full length protein

1:800

Millipore (Temecula,

CA, USA)

Iba1/ rabbit

Anti-Ionized calcium binding adaptor molecule 1/ 019-19741

Synthetic peptide (Iba1 C-terminal sequence)

1:750

WAKO (Richmond,

VA, USA)

mCherry/ rabbit

Anti-mCherry antibody/ AB167453

mCherry

1:1000

1:1000

Abcam (Cambridge,

UK)

NeuN/ mouse

Anti-NeuN Antibody, clone A60 / MAB377

1:1000

Millipore (Temecula,

CA, USA)

PSD95/mouse

PSD95 clone K28/43/75–028

77–299 (PDZ

domains 1 and 2)

1:1000 UC

Davis/NIH NeuroMab Facility (Davis, CA, USA)

TH/ mouse

Anti-Tyrosine Hydroxylase

clone LNC1 /MAB318

Recognizes an epitope on the outside of the regulatory N-terminus

1:1000

Millipore (Temecula,

CA, USA)

Synpato-physin/

rabbit

Synaptophysin/PA1–1043

253–272 1:50000–

1:25000

Invitrogen (Waltham, MA, USA)

Secondary antibodies

IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody

680RD-conjugated goat anti-rabbit/ 926–68,071

1:20000

LI-COR Biosciences

(Lincoln, NE, USA)

IRDye 800CW Goat

anti-Rabbit IgG

Secondary Antibody

800CW-conjugated goat anti-rabbit/ 926–32,211

1:20000

LI-COR Biosciences

(Lincoln, NE, USA)

IRDye 680RD Goat anti-Mouse IgG Secondary Antibody

680RD-conjugated goat anti-mouse/ 926–68,070

1:20000

LI-COR Biosciences

(Lincoln, NE, USA)

IRDye 800CW Goat

anti-Mouse IgG

Secondary Antibody

800CW-conjugated goat anti-mouse/ 926–32,210

1:20000

LI-COR Biosciences

(Lincoln, NE, USA)

Goat Anti-Mouse IgG Antibody (H + L), Biotinylated

Biotinylated Goat Anti-mouse/ BA-9200

1:500

Vector Laboratories

(Burlingame, CA, USA)

Alexa Fluor 488 goat anti-rabbit (H + L)

Alexa Fluor 488 goat anti-rabbit/ A-11,008

1:1000

Invitrogen (Waltham,

MA, USA)

Alexa Fluor 633 goat anti-rabbit (H + L)

Alexa Fluor 633 goat anti-rabbit/ A21071

1:1000

Invitrogen (Waltham,

MA, USA)

Alexa Fluor 488 goat anti-mouse (H + L)

Alexa Fluor 488 goat anti-mouse/ A-11,029

1:1000

Invitrogen (Waltham,

MA, USA)

Alexa Fluor 633 goat anti-mouse (H + L)

Alexa Fluor 633 goat anti-mouse/

A-21,052

1:1000

Invitrogen (Waltham,

MA, USA)

Cy™3 AffiniPure Goat Anti-Rabbit IgG (H + L)

111-165-003

1:1000

Jackson ImmunoResearch Laboratories, Inc

872 West Baltimore Pike

West Grove, Pennsylvania 19,390

USA