Fig. 2
From: NMNAT2 supports vesicular glycolysis via NAD homeostasis to fuel fast axonal transport
Deleting NMNAT2 in cortical glutamatergic neurons results in Amyloid Precursor Protein (APP) accumulation (A, B, C) Representative confocal microscopy images showing APP accumulation in multiple brain regions of ctrl and cKO mice at P5 and P21. Scale bars, 50 um for all left panels, 100 um for all right panels. Dashed white lines mark the margins of corpus callosum (A) and fimbria (B). D High magnification image showing that the accumulated APP is encircled by myelin sheath labeled by myelin basic protein (MBP) staining in P16 cKO corpus callosum (observed in 3 out of 3 cKO brains). E Quantification of the APP signal in the corpus callosum, hippocampal fimbria, and striatum area of cKO mice normalized to the average signal in ctrl mice at P5 and P21. Number of mice: P5, 3–6 ctrl, and 3–6 cKO; P16/P21 3–6 ctrl and 3–4 cKO. Unpaired t-test and Mann–Whitney test were applied for the statistic result, *p < 0.05, **p < 0.01, ***p < 0.001