Fig. 7

Deleting SARM1 prevents neurodegenerative phenotypes in NMNAT2 cKO brains. A Bright field images of coronal brain sections (rostral to caudal from left to right) from ctrl, NMNAT2 cKO; S^null/ + , and NMNAT2 cKO; S^null/S^null mice. Enlarged ventricle, greatly reduced corpus callosum, and internal capsule were evident in NMNAT2 cKO; S^null/ + but not in NMNAT2 cKO; S^null/S^null brains. Abbreviations: Ac, anterior commissure; Ic, internal capsule; CC, corpus callosum; S1-Cx, primary somatosensory cortex; Hi, hippocampus; St, striatum; VC, ventricle. B Thickness of the primary somatosensory cortex in P16/21 and P90 mice. Number of mice: P16/21, 8 ctrl, 11 NMNAT2 cKO; S^null/ + , 6 NMNAT2 cKO; S^null/S^null; P90, 5 ctrl, 6 NMNAT2 cKO; S^null/ + , 4 NMNAT2 cKO; S^null/S^null. ***, p = 0.0018, q = 0.0009. (Ctrl vs. NMNAT2 cKO; S^null/ +) *, p = 0.0049, q = 0.0103. (NMNAT2 cKO; S^null/ + vs. NMNAT2 cKO; S^null/S^null) *, p = 0.0341, q = 0.0358. C Representative NF-M (black) immunostaining images showing corpus callosum around the midline area in ctrl, NMNAT2 cKO; S^null/ + , and NMNAT2 cKO; S^null/S^null rostral brain section at P21. D CC thickness quantification. Number of mice: P21, 10 ctrl, 8 NMNAT2 cKO; S^null/ + , 5 NMNAT2 cKO; S^null/S^null. E Immunohistology images showing APP accumulation in the corpus callosum, fimbria, and striatum of P21 NMNAT2 cKO; S^null/ + , but not in ctrl and NMNAT2 cKO; S^null/S^null brains. F Quantification of the APP signal in the brain regions shown in (E) normalized to the value in ctrl mice. Number of mice: 7–9 ctrl, 4–5 NMNAT2 cKO; S^null/ + , 4 NMNAT2 cKO; S^null/S^null. CC, corpus callosum; Str, striatum; Fim, Fimbria. (Fim Ctrl vs. NMNAT2 cKO; S^null/ +) **, p = 0.0186, q = 0.0098; (NMNAT2 cKO; S^null/ + vs. NMNAT2 cKO; S^null/S^null) **, p = 0.0186, q = 0.0098. All above data represent mean ± SEM. One-way ANOVA with a two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli was used, ****p < 0.0001