Fig. 3
From: Amyloid-β specific regulatory T cells attenuate Alzheimer’s disease pathobiology in APP/PS1 mice
Adoptive transfer of TCRAβ-Tregs improve memory function in APP/PS1 mice. A. Experimental timeline of adoptive transfer experiments. Eight-month-old non-Tg mice (n = 6) were untreated, and age-matched APP/PS1 mice were untreated or treated with 1 × 106 TCRAβ-Tregs, polyclonal Tregs (Treg), or EV-Tregs (TCR−−-Tregs electroporated with empty plasmid vector). B Y maze test performed on experimental mice after adoptive transfers to evaluate spontaneous alteration in mice freely exploring each arm of the Y maze over eight minutes. Successful entries were defined as consecutive entries into three different arms and the number of entries/mice were recorded (n = 6). C. Radial arm water maze (RAWM) test performed with experimental mice after adoptive transfers. After four trials (T1-T4) the mice were returned their cages for 30 min and reintroduced into the T4 arm for the delayed retention trial (T5). Each trial lasted for 1 min and errors were scored when the mice entered the wrong arm or entered the arm without climbing the platform or didn’t make a choice for 20 s. The trial ended when the mice climbed and stayed on platform for at least 10 s. Errors of 9-day trial were divided into three blocks: Block-1 (days 1–3), Block-2 (days 4–6), Block-3 (days 7–9). The errors in each block were averaged for statistical analysis (n = 6). D Representative 18F-FDG PET images of brain glucose uptake in different treatment groups on the day of sacrifice. E Quantification of 18F-FDG PET brain glucose uptake on the day of sacrifice (n = 5—6). B, C, and E Data presented as mean ± SEM. One-way ANOVA followed by Turkey’s post hoc test was used to determine significant differences between experimental groups. *p < 0.05, **p < 0.01, ***p < 0.001