Fig. 5
From: Amyloid-β specific regulatory T cells attenuate Alzheimer’s disease pathobiology in APP/PS1 mice
Adoptive transfer of TCRAβ-Tregs reduces amyloid load in APP/PS1 mice. APP/PS1 mice were untreated or treated with TCRAβ-Tregs, polyclonal Tregs (Treg) or EV-Tregs (TCR−−-Tregs electroporated with empty plasmid vector) by adoptive transfer and brain tissues acquired 3 weeks post adoptive transfer A. ELISA performed to quantify Aβ1–42 levels in the brain using Tris–HCl (soluble) and guanidine-HCL (insoluble) fractions of cortical tissue. B, C. ImmunohistoFchemistry (pan-Aβ) and immunofluorescence (Thioflavin-S) to determine the area occupied by insoluble Aβ plaques in cortical and hippocampal regions. D Representative images showing amyloid plaque (pan-Aβ) and Thioflavin-S staining in different brain regions. Percent area occupied was quantified using Cavalieri estimator probe. Scale bar = 100 µm. A-C Data presented as mean ± SEM for n = 5–6 mice/group. One-way ANOVA followed by Turkey’s post hoc test was used to determine significant differences between experimental groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001