Fig. 7

Molecular similarities between tyrosine hydroxylase (TH) enriched cell types depleted in the substantia nigra pars compacta of PD patients. A TH expression comparison between dopaminergic Neurons0 and TH enriched glial cell types (Astrocytes2, Microglia1 and Oligos2). Higher level cell markers were used to confirm the identity of these subpopulations. B Venn diagram showing overlap of cell markers defining dopaminergic Neurons0 and TH enriched glial cell types. C Gene expression levels of cell markers shared between dopaminergic Neurons0 and TH enriched glial cell types. D GO term pathway analysis on the shared cell markers. E Representative images from spatial transcriptomics experiments showing the presence of SNAP25, SLC6A3 and TH transcripts (dots) in higher-order cell types (represented as polygons). Astro, astrocytes; VC, vascular cells; Micro, microglia; Oligo, oligodendrocytes; OPC, oligodendrocyte progenitor cells. Scale bar, 50 µm. F Gene expression levels for shared cell markers of higher-order cell types determined by spatial transcriptomics. Violin plots of SCT-normalized data are shown