Fig. 5

GV-971 significantly altered microglia inflammatory activation status surrounding Aβ plaques. a Representative immunofluorescent images of P2ry12⁺ microglia (green) and Clec7a⁺ microglia (red) clustering around 3D6⁺ Aβ plaque (blue) in mice from University of Chicago. b Quantification of the average number of cortical Clec7a⁺ cells within 0.02 mm2 area of 3D6⁺ Aβ plaque in cortices of APPPS1-21 mice treated with 40 mg/kg, 80 mg/kg, or 160 mg/kg GV-971 or vehicle (male n = 12–9, female n = 9–11). c Quantification of the average number of cortical P2ry12⁺ cells within 0.02 mm2 area of 3D6⁺ Aβ plaque in cortices of APPPS1-21 mice treated with 40 mg/kg, 80 mg/kg, or 160 mg/kg GV-971 or vehicle (male n = 12–9, female n = 9–11). Data are presented as mean SEM. Significance was determined using One-way ANOVA test followed by Tukey’s multiple comparison post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001