Fig. 5

Loss of Cisd promotes mitophagy flux. A, B Confocal microscopy analysis of mitophagy reporter mito-QC in flight muscle from WT control and Cisd knockdown flies driven by Mef2-GAL4 of the indicated ages. B Quantification of the number of mitolysosomes shown in A. Data points indicated individual animals analysed. Statistical analysis: unpaired t-test; *P < 0.05; **P < 0.01. C Immunoblot analysis of ARPE-19 cells expressing mito-QC, shown in D, with non-targeting siRNAs (Ctrl) or targeting CISD1, CISD2 or both. D Confocal microscopy analysis of mitophagy using mito-QC in cells shown in C. E Quantification of the number of mitolysosomes shown in D. Data points indicate replicate experiments. Statistical analysis: one-way ANOVA with Dunnett’s post-hoc correction; *P < 0.05; **P < 0.01. Scale bars = 10 μm