Fig. 6

Cisd knockdown ameliorates Pink1/parkin mutant phenotypes. A, B Climbing analysis of WT control versus (A) parkin or (B) Pink1 mutants with control or Cisd RNAi. GD and KK indicate independent RNAi lines driven by da-GAL4. See also Fig. S 3B. N is shown inside bars. C, D Confocal microscopy of flight muscle from (young) wild-type control (WT ctrl) or (C) parkin or (D) Pink1 mutants expressing control or Cisd RNAi stained for the mitochondrial marker ATP5a. E Quantification of dopaminergic (DA) neurons shown in F. F 30-day-old WT or parkin or Pink1 mutants expressing control or Cisd RNAi, immunostained for tyrosine hydroxylase. G, H Lifespan analysis of WT versus (G) parkin or (H) Pink1 mutants with control or Cisd RNAi. Statistical analyses: (A, B) Kruskal-Wallis non-parametric test with Dunn’s post-hoc correction, (E) one-way ANOVA with Sidak’s post-hoc correction; (G, H) Log rank (Mantel-Cox) test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Scale bars = 10 μm