Fig. 4

Inhibition of the MCU prevents neurons from mitochondrial oxidative stress. a Representative pictures of the effects of the inhibition of the MCU on the mitochondrial oxidative stress induced by TgCM in neurons. Scale bar represents 20 μm and 5 μm in the insets. After exposure to Ru360, TgCM not was able to increase oxidative stress in mitochondria. b—d Histograms of mitochondrial oxidative stress frequency distribution (Ratio 800/900) before and after application of TgCM with or without Ru360, and of Ru360 alone. e Averaged Ratios 800/900 before and after TgCM treatment with or without Ru360, and Ru360 alone for each z-stack acquired. Darker traces represent z-stacks showing an increase ≥ 25% in 800/900 ratio after topical application of TgCM or Ru360. f Scatter dot plot shows the relative change (ΔR/Ro) in ratio 800/900 for each condition. Error bars represent mean ± SEM (TgCM: 32.50 ± 3.328%, 24 z-stack; TgCM + Ru360: 13.64 ± 3.187%, 17 z-stacks; Ru360: 11.14 ± 2.660%, 14 z-stacks from 4, 4 and 3 mice respectively (all male), **p < 0.01, ***p < 0.001)