Fig. 2

Performance of protein pairs in distinguishing A+T+ and A-T- individuals. A SVM modelling results showed as boxplots ordered based on median ROC AUC. In the modelling, levels of the two CSF proteins were used as the predictor variables and the amyloid and tau status as a response variable, with only SCD individuals included in the A-T- sample group. Each boxplot corresponds to one protein pair and includes the results from 101 repeated models. Boxplot whiskers and outlying data points were removed for visualisation purposes. B A heatmap of the median ROC AUC values from SVM models from each protein pair. The heatmap is clustered based on the ROC AUC results and annotated on the left with clustering results from Fig. 1A, based on the correlation of the individual proteins to amyloid beta peptides, tau and NfL markers in A+T+ individuals. C, D ROC curves constructed from the best protein pair (GAP43 and PTPRN2) median model predictions for discovery cohort (C) and validation cohort (D) (left). The relationship between the CSF levels of GAP43 and PTPRN2 showed as scatterplots with datapoints (individual samples) colored based on amyloid and tau status in discovery (C) and validation (D) cohort (right). The relationship is further visualized using robust linear regression