Fig. 5
From: A partial Drp1 knockout improves autophagy flux independent of mitochondrial function
RNAseq reveals Mn impairs autophagy but not mitochondrial pathways in vivo. C57/BL6 mice (3–4-month-old) were orally gavaged with MnCl2 4H2O (15 mg/kg/day) or water control for 30 consecutive days before harvesting (n = 8 for each group). Total RNA was extracted from the ventral midbrain and processed for RNAseq. (A) Volcano plot showing the number of differentially expressed genes between the Mn-treated mice and their control littermates (> one-fold change, p < 0.05). (B) Heat map showing differential expressed genes (DEGs) of the autophagy pathways between the Mn-treated mice and the control mice. (C) Genes related to mitochondrial dynamics were not significantly affected after Mn exposure [-log10(p-value) < 1.301]. (D) Genes involved in mitochondrial function show 97.7% (128/131 genes) were not significantly affected in the Mn-treated mice compared to the control group [-log10(p-value) < 1.301]. For brevity, only the top 50 genes are shown here. Additional genes are included in the Supplementary file 1