Fig. 6
From: A partial Drp1 knockout improves autophagy flux independent of mitochondrial function
Selective autophagy impairment induced by Mn in the ventral midbrain. Autophagy reporter mice [C57BL/6-Tg(CAG-RFP/EGFP/Map1lc3b)1Hill/J] were treated with MnCl2 4H2O (15 mg/kg/day) or water through oral gavage once daily for 30 consecutive days, then perfused for immunostaining and confocal imaging of DA neurons (A), tyrosine hydroxylase-positive and GABA neurons (B), GAD67-positive. Autophagic vesicles were quantified for autophagosome and autolysosome as described in Fig. 1 and normalized as per 100 µm2 for DA neurons (C) and GABA neurons (D) The conversion rate of autophagosome to autolysosome was calculated by expressing the ratio of these two types of vesicles (E). Significant difference between control and Mn treatment group was not observed in GABA neurons (P = 0.1257). Data represent Mean ± SEM, n = 5 per group, with approximately 40–50 neurons analyzed per animal. The number of autophagosomes, autolysosomes, and their ratio were compared using an independent t-test