Fig. 1

β-glucocerebrosidase is upregulated upon myelination induction in vitro. a Representative western blot of protein extracts from Oli-neu cells untreated or treated for 1, 2 or 3 days with dbcAMP (t0, t1, t2 and t3, respectively). MAG, CNP and PLP myelin protein levels were analyzed; β-actin (ACTB) was used as loading control. b Densitometric quantification of western blots as in (a), showing expression of CNP1, PLP and MAG myelin proteins in Oli-neu cells treated with dbcAMP (*, p < 0.05; Kruskal–Wallis test and Dunn's test for multiple comparison, n = 3 independent experiments). Error bars indicate s.e.m. c qRT-PCR analysis of Gba1 and Gba2 mRNA from Oli-neu cells treated with dbcAMP for three days. Actb was used as housekeeping gene (*, p < 0.05; Kruskal–Wallis test and Dunn's test for multiple comparison, n = 3 independent experiments). Error bars indicate s.e.m. d Representative western blot of GBA1 protein levels in Oli-neu cells upon dbcAMP treatment. Vinculin (VNC) was used as loading control. e Densitometric quantification of western blot as in (d) (*, p < 0.05; **, p < 0.001; unpaired two-tailed Student’s t test; n = 3 independent experiments). Error bars indicate s.e.m. f Enzymatic activity assay performed on protein extracts from Oli-neu cells treated with dbcAMP (*, p < 0.05; **, p < 0.01; Kruskal–Wallis test and Dunn's test for multiple comparison; n = 5 independent experiments). Error bars indicate s.e.m. g Representative western blot of LAMP1 and IDS protein levels in Oli-neu cells treated with dbcAMP. ACTB was used as loading control. h Densitometric quantification of western blot as in (g) (*, p < 0.05; Kruskal–Wallis test and Dunn's test for multiple comparison, n = 4 independent experiments). Error bars indicate s.e.m