Fig. 2

APOE4 Microglia exhibit lower spontaneous motility in the entorhinal cortex. A Confocal images of microglia spontaneous motility time-lapse at different time points, and binary overlap. Scale bar 10 µm. B Binary overlaps of APOE3 and APOE4 microglia in the EC and CA1. Quantification of microglial baseline processes movement (motility index = green pixels + red pixels/yellow pixels) in the EC (C) and CA1 (D). Bars represent the average motility index of all analyzed microglia ± SEM, and symbols (circles, male; triangle, females) represent data points for each microglia (3–4 animals per genotype). ***p < 0.001; unpaired two-tailed Student’s t test