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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: APOE4 genotype and aging impair injury-induced microglial behavior in brain slices, including toward Aβ, through P2RY12

Fig. 5

P2RY12 is downregulated in APOE4 microglia. A Confocal images of GFP (green) P2RY12 (red) in APOE3 (E3) and APOE4 (E4) brains. P2RY12 is expressed mainly in the membrane of microglial processes distal from microglial soma. Scale bar 60 µm. B Quantification of P2RY12 labeling intensity (arbitrate units, see methods) in total field. Individual points represent each animal. 2 brain slices per animal, 4 animals (males) per genotype. *p < 0.05, two-tailed student t-test. C Quantification of percent P2RY12 in microglial processes. Individual points represent individual fields. 2 fields per brain slice, 2 brain slices per animal, 4 animals (males) per genotype. *p < 0.05, two-tailed student t-test. Fold change levels of P2RY12 mRNA. RNA was extracted from hemibrain (6 animals per genotype). Level of transcript was determined through double delta CT

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Molecular Neurodegeneration

ISSN: 1750-1326