Fig. 6

APOE4 microglia exhibit slower response to Aβ peptides, which requires P2RY12. A Representative confocal zt-stacks showing the time course of microglial response (green) to Hi-Lyte Fluor 555-labeled Aβ (Aβ − 42) (red) in acute entorhinal slices. Scale bar 10 µm. B Percent coverage computed as the percent area of infused Aβ − 42 covered by microglial processes over time. Mean percent coverage ± SEM plotted. Two-way ANOVA with Sidak’s multiple comparison post-hoc analyses to show the effect of APOE genotype, and two-tailed student t-test to compare at each time point. C 3D reconstruction of microglial processes internalizing Aβ 6 h post-injection. Scale bar 10 µm. D Acute brain slices were incubated in 10 µM PSB. Representative confocal zt-stacks showing the time course of microglial response (green) to Hi-Lyte Fluor 555-labeled Aβ (Aβ − 42) (red). Scale bar 10 µm